Mitochondrial DNA and filtering of contaminated sequences
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چکیده
We analyzed a total of 27,958,007 sequences that all had perfect matches to the expected indices (Extended Data Table 6). We merged the reads from either end of the same molecule by requiring an overlap of at least 11 base pairs, and generated a consensus sequence by picking the base with the higher sequence quality. We mapped these merged reads, which we call a “fragment”, to the revised Cambridge reference sequence (rCRS NC_012920). We restricted analysis to Oase 1 fragments that were at least 35bp and that had a mapping quality of at least 30. We removed duplicate fragments by identifying all fragments with the same start and stop positions and keeping the one with the highest average base quality.
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